Journal: bioRxiv

Article Title: Outer membrane vesicles derived from Salmonella Typhimurium can deliver Shigella flexneri 2a O-polysaccharide antigen to prevent lethal pulmonary infection in mice

doi: 10.1101/2021.02.01.429291

Figure Lengend Snippet: Splenocytes were isolated from 9 mice immunized intranasally or by intraperitoneal administration. Levels of cytokines were measured to evaluate the Th1/Th2 polarization effect. These data display the levels of cytokines in the splenocytes of mice immunized by intranasal or intraperitoneal administration with an OMV vaccine, OMV vector and PBS. After stimulation of the splenocytes with 6μg/ml LPS isolated from S. flexneri for 24 hours, levels of IFN-γ (A), IL-12 (p40) (B), IL-4 (C), IL-13 (D), IL-6 (E) and TNF-α (F) were measured by quantitative ELISA from the supernatants. Error bars represent variations between mice in each group. P < 0.01 compared with mice immunized with Salmonella -derived OMV vector (Negative control).

Article Snippet: The wells were washed and incubated with biotinylated monoclonal anti-INF-γ, anti-IL-12 (p40), anti-IL-4, anti-IL-13, anti-IL-6, or anti-TNF-α antibodies (BD Biosciences, Billerica, Massachusetts, USA).

Techniques: Isolation, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Derivative Assay, Negative Control